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Glucose 40 g
Yeast extract 10 g
Peptone 10 g
Na2HPO4.12H2O 3.38 g
Citric acid.H2O 1.5 g
Ethanol 20 ml
Acetic acid 10 ml
Agar (if needed) 15 g
Distilled water 970 ml
Sterilize ethanol and acetic acid separately by filtration.
Glucose 10 g
Yeast extract 2 g
Peptone 3 g
Agar (if needed) 15 g
Acetic acid (100%) 10 ml
Ethanol (96%) 20 ml
Distilled water 1000 ml
Sterlize ethanol and acetic acid separately by filtration and add aseptically to the sterile medium.