Collection TBRC
Other code 3-44-a(19)
Other collection code BCC 72027 ; DSM 45348 ; JCM 14710 ; MTCC 9007
History <---- BCC 72027 <---- DSM 45348 <---- JCM 14710 <---- I. Ara and T. Kudo; 3-44-a(19)
Microorganism group Bacteria
Type strain Yes
Ara I, Matsumoto A, Bakir MA, Kudo T, Omura S, Takahashi Y. Pseudosporangium ferrugineum gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1644-52.
LPSN
Source Soil - sandy soil
Country of origin Bangladesh
Risk Group 1
Growth medium

Trypticase soy broth (Difco) 30 g
Agar 15 g
Distilled water 1000 ml

Yeast extract 4 g
Malt extract 10 g
Dextrose (d-glucose) 4 g
Agar (if needed) 15 - 20 g
Distilled water 1000 ml

CaSO4.2H2O 0.5 g
Ca(NO3)2.4H2O 0.25 g
MgSO4.7H2O 0.05 g
K2SO4 0.03 g
KH2P04 0.02 g
NaHCO3 0.1 g
CaCl2.2H2O 0.02 g
Yeast extract 0.1 g
Casamino acids 0.1 g
Glucose 0.2 g
Soil extract* 100 ml
Trace element mix 1** 0.3 ml
Vitamin mix A1*** 1 ml
Agar (if needed) 18 g
Distilled water 900 ml
Add vitamin mix A1 after sterilization and cooling down at 50C.
* Humic soil 1000 g
Distilled water 1000 ml
After sterilization, collect liquid portion by filtration/centrifigation. Soil extract can be stored at 4C for further use.
** CaCl2.2H2O 4 g
ZnSO4.7H2O 2 g
Na2B4O7.10H2O 0.1 g
FeSO4. 7H2O 5 g
KJ 0.05 g
CoCl2.6H2O 0.5 g
CuSO4.5H2O 0.2 g
MnCl2.4H2O 2 g
Na2MoO4.2H2O 0.05 g
H2SO4 95 - 97 % p.a. 1 ml
Demineralized water 1000 ml
***p-aminobenzoic acid 0.05 g
Calcium pantothenate 0.05 g
Inositol 0.05 g
Niacin 0.05 g
Pyridoxin HCl 0.05 g
Riboflavin 0.05 g
Thiamine HCl 0.05 g
Biotin 0.025 g
Demineralized water 100 ml
Sterilize by filtration

Growth temperature 25-30 °C
Oxygen requirement Aerobic
Distribution condition TBRC MTA
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