TBRC | Pseudosporangium ferrugineum (TBRC 3949)

Collection	TBRC
Other code	3-44-a(19)
Other collection code	BCC 72027 ; DSM 45348 ; JCM 14710 ; MTCC 9007
History	<---- BCC 72027 <---- DSM 45348 <---- JCM 14710 <---- I. Ara and T. Kudo; 3-44-a(19)
Microorganism group	Bacteria
Type strain	Yes [712] Ara I, Matsumoto A, Bakir MA, Kudo T, Omura S, Takahashi Y. Pseudosporangium ferrugineum gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1644-1652.
LPSN
Source	Soil - sandy soil
Country of origin	Bangladesh
Risk Group	1
Growth medium	TSA Trypticase soy broth (Difco) 30 g Agar 15 g Distilled water 1000 ml ISP2 Yeast extract 4 g Malt extract 10 g Dextrose (d-glucose) 4 g Agar (if needed) 15 - 20 g Distilled water 1000 ml Soil extract agar CaSO4.2H2O 0.5 g Ca(NO3)2.4H2O 0.25 g MgSO4.7H2O 0.05 g K2SO4 0.03 g KH2P04 0.02 g NaHCO3 0.1 g CaCl2.2H2O 0.02 g Yeast extract 0.1 g Casamino acids 0.1 g Glucose 0.2 g Soil extract* 100 ml Trace element mix 1 0.3 ml Vitamin mix A1* 1 ml Agar (if needed) 18 g Distilled water 900 ml Add vitamin mix A1 after sterilization and cooling down at 50C. * Humic soil 1000 g Distilled water 1000 ml After sterilization, collect liquid portion by filtration/centrifigation. Soil extract can be stored at 4C for further use. CaCl2.2H2O 4 g ZnSO4.7H2O 2 g Na2B4O7.10H2O 0.1 g FeSO4. 7H2O 5 g KJ 0.05 g CoCl2.6H2O 0.5 g CuSO4.5H2O 0.2 g MnCl2.4H2O 2 g Na2MoO4.2H2O 0.05 g H2SO4 95 - 97 % p.a. 1 ml Demineralized water 1000 ml *p-aminobenzoic acid 0.05 g Calcium pantothenate 0.05 g Inositol 0.05 g Niacin 0.05 g Pyridoxin HCl 0.05 g Riboflavin 0.05 g Thiamine HCl 0.05 g Biotin 0.025 g Demineralized water 100 ml Sterilize by filtration
Growth temperature	25-30 °C
Oxygen requirement	Aerobic
Distribution condition	TBRC MTA
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