Jojima T, Ioku Y, Fukuta Y, Shirasaka N, Matsumura Y, Mori M. Collibacillus ludicampi gen. nov., sp. nov., a new soil bacterium of the family Alicyclobacillaceae. Int J Syst Evol Microbiol. 2023 May;73(5):005827.
Solution A
Yeast extract 1 g
(NH4)2SO4 0.2 g
MgSO4 7H2O 1.00 g
CaCl2 2H2O 0.25 g
KH2PO4 0.6 g
Distilled water 500 ml
pH to 4.5
Solution B
Glucose 1 g
Gellan gum 8 g
Distilled water 500 ml
Sterilize solution A and B separately at 121?C for 15 min. Cool to 60?C then mix A and B.