TBRC | Rhodoferax mekongensis (TBRC 17307)

Collection	TBRC
Other code	D8-11
Other collection code	NBRC 116415
History	<---- P. Rattanawaree, D8-11 <---- S. Kitikhun, D8-11
Microorganism group	Bacteria
Type strain	Yes [1360] Kitikhun S, Charoenyingcharoen P, Siriarchawatana P, Likhitrattanapisal S, Nilsakha T, Chanpet A, Jeennor S, Yukphan P, Ingsriswang S. Rhodoferax potami sp. nov. and Rhodoferax mekongensis sp. nov., isolated from the Mekong River in Thailand. Int J Syst Evol Microbiol. 2024 May;74(5):006351.
LPSN
Source	Water
Country of origin	Thailand
Risk Group	1
Growth medium	NA Peptone 5 g Beef extract 3 g Agar (if needed) 20 g Distilled water 1000 ml pH 7 LB Tryptone 10 g Yeast extract 5 g NaCl 10 g Agar (if needed) 15 g Distilled water 1000 ml pH 7.5 Soil extract agar CaSO4.2H2O 0.5 g Ca(NO3)2.4H2O 0.25 g MgSO4.7H2O 0.05 g K2SO4 0.03 g KH2P04 0.02 g NaHCO3 0.1 g CaCl2.2H2O 0.02 g Yeast extract 0.1 g Casamino acids 0.1 g Glucose 0.2 g Soil extract* 100 ml Trace element mix 1 0.3 ml Vitamin mix A1* 1 ml Agar (if needed) 18 g Distilled water 900 ml Add vitamin mix A1 after sterilization and cooling down at 50C. * Humic soil 1000 g Distilled water 1000 ml After sterilization, collect liquid portion by filtration/centrifigation. Soil extract can be stored at 4C for further use. CaCl2.2H2O 4 g ZnSO4.7H2O 2 g Na2B4O7.10H2O 0.1 g FeSO4. 7H2O 5 g KJ 0.05 g CoCl2.6H2O 0.5 g CuSO4.5H2O 0.2 g MnCl2.4H2O 2 g Na2MoO4.2H2O 0.05 g H2SO4 95 - 97 % p.a. 1 ml Demineralized water 1000 ml *p-aminobenzoic acid 0.05 g Calcium pantothenate 0.05 g Inositol 0.05 g Niacin 0.05 g Pyridoxin HCl 0.05 g Riboflavin 0.05 g Thiamine HCl 0.05 g Biotin 0.025 g Demineralized water 100 ml Sterilize by filtration
Growth temperature	30 °C
Oxygen requirement	Aerobic
Distribution condition	TBRC MTA
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Overseas: 3,000 Baht

Thailand: 2,000 Baht

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