Carro L, Oren A. Descriptions of Micromonospora grosourdyae nom. nov., Micromonospora sonchi comb. nov. and Micromonospora thawaii sp. nov. to resolve problems with the taxonomy and nomenclature of strains named Micromonospora endophytica. Int J Syst Evol Microbiol. 2022;72(12):005628.
CaSO4.2H2O 0.5 g
Ca(NO3)2.4H2O 0.25 g
MgSO4.7H2O 0.05 g
K2SO4 0.03 g
KH2P04 0.02 g
NaHCO3 0.1 g
CaCl2.2H2O 0.02 g
Yeast extract 0.1 g
Casamino acids 0.1 g
Glucose 0.2 g
Soil extract* 100 ml
Trace element mix 1** 0.3 ml
Vitamin mix A1*** 1 ml
Agar (if needed) 18 g
Distilled water 900 ml
Add vitamin mix A1 after sterilization and cooling down at 50C.
* Humic soil 1000 g
Distilled water 1000 ml
After sterilization, collect liquid portion by filtration/centrifigation. Soil extract can be stored at 4C for further use.
** CaCl2.2H2O 4 g
ZnSO4.7H2O 2 g
Na2B4O7.10H2O 0.1 g
FeSO4. 7H2O 5 g
KJ 0.05 g
CoCl2.6H2O 0.5 g
CuSO4.5H2O 0.2 g
MnCl2.4H2O 2 g
Na2MoO4.2H2O 0.05 g
H2SO4 95 - 97 % p.a. 1 ml
Demineralized water 1000 ml
***p-aminobenzoic acid 0.05 g
Calcium pantothenate 0.05 g
Inositol 0.05 g
Niacin 0.05 g
Pyridoxin HCl 0.05 g
Riboflavin 0.05 g
Thiamine HCl 0.05 g
Biotin 0.025 g
Demineralized water 100 ml
Sterilize by filtration