TBRC | Micromonospora endophytica (TBRC 3312)

Collection	TBRC
Other code	DCWR-9-8-2
Other collection code	BCC 67267 ; NBRC 110008
History	<---- BCC 67267 <---- C. Thawai, DCWR-9-8-2
Microorganism group	Bacteria
Type strain	Yes [1030] Thanaboripat D, Thawai C, Kittiwongwattana C, Laosinwattana C, Koohakan P, Parinthawong N. Micromonospora endophytica sp. nov., an endophytic actinobacteria of Thai upland rice (Oryza sativa). J Antibiot (Tokyo). 2015;68:680-684. [1275] Carro L, Oren A. Descriptions of Micromonospora grosourdyae nom. nov., Micromonospora sonchi comb. nov. and Micromonospora thawaii sp. nov. to resolve problems with the taxonomy and nomenclature of strains named Micromonospora endophytica. Int J Syst Evol Microbiol. 2022;72(12):005628.
LPSN
Source	Tissue (Oryza sativa)
Country of origin	Thailand
Risk Group	1
Growth medium	ISP2 Yeast extract 4 g Malt extract 10 g Dextrose (d-glucose) 4 g Agar (if needed) 15 - 20 g Distilled water 1000 ml Yeast soluble starch agar Yeast extract 2 g Soluble starch 10 g Agar (if needed) 15 g Distilled water 1000 ml pH 7.3 Soil extract agar CaSO4.2H2O 0.5 g Ca(NO3)2.4H2O 0.25 g MgSO4.7H2O 0.05 g K2SO4 0.03 g KH2P04 0.02 g NaHCO3 0.1 g CaCl2.2H2O 0.02 g Yeast extract 0.1 g Casamino acids 0.1 g Glucose 0.2 g Soil extract* 100 ml Trace element mix 1 0.3 ml Vitamin mix A1* 1 ml Agar (if needed) 18 g Distilled water 900 ml Add vitamin mix A1 after sterilization and cooling down at 50C. * Humic soil 1000 g Distilled water 1000 ml After sterilization, collect liquid portion by filtration/centrifigation. Soil extract can be stored at 4C for further use. CaCl2.2H2O 4 g ZnSO4.7H2O 2 g Na2B4O7.10H2O 0.1 g FeSO4. 7H2O 5 g KJ 0.05 g CoCl2.6H2O 0.5 g CuSO4.5H2O 0.2 g MnCl2.4H2O 2 g Na2MoO4.2H2O 0.05 g H2SO4 95 - 97 % p.a. 1 ml Demineralized water 1000 ml *p-aminobenzoic acid 0.05 g Calcium pantothenate 0.05 g Inositol 0.05 g Niacin 0.05 g Pyridoxin HCl 0.05 g Riboflavin 0.05 g Thiamine HCl 0.05 g Biotin 0.025 g Demineralized water 100 ml Sterilize by filtration
Growth temperature	25-30 °C
Oxygen requirement	Aerobic
Distribution condition	TBRC MTA
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Overseas: 3,000 Baht

Thailand: 2,000 Baht

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