TBRC | Sphaerimonospora cavernae (TBRC 1851)

Collection	TBRC
Other code	N74
Other collection code	BCC 77604 ; NBRC 11148
History	<---- BCC 77604 <---- K. Duangmal, N74
Microorganism group	Bacteria
Type strain	Yes [916] Mingma R, Duangmal K, Tak? A, Inahashi Y, Omura S, Takahashi Y, Matsumoto A. Proposal of Sphaerimonospora cavernae gen. nov., sp. nov. and transfer of Microbispora mesophila (Zhang et al., 1998) to Sphaerimonospora mesophila comb. nov. and Microbispora thailandensis (Duangmal et al., 2012) to Sphaerimonospora thailandensis comb. nov. Int J Syst Evol Microbiol. 2016 Apr;66(4):1735-1744.
LPSN
Source	Soil - cave
Country of origin	Thailand
Risk Group	1
Growth medium	ISP2 Yeast extract 4 g Malt extract 10 g Dextrose (d-glucose) 4 g Agar (if needed) 15 - 20 g Distilled water 1000 ml ISP3 Oatmeal 20 g Trace salts solution* 1 ml Agar (if needed) 18 g Distilled water 1000 ml pH 7.2 * FeSO4.7H2O 0.1 g MnCl2.4H2O 0.1 g ZnSO4.7H2O 0.1 g Distilled water 100 ml Soil extract agar CaSO4.2H2O 0.5 g Ca(NO3)2.4H2O 0.25 g MgSO4.7H2O 0.05 g K2SO4 0.03 g KH2P04 0.02 g NaHCO3 0.1 g CaCl2.2H2O 0.02 g Yeast extract 0.1 g Casamino acids 0.1 g Glucose 0.2 g Soil extract* 100 ml Trace element mix 1 0.3 ml Vitamin mix A1* 1 ml Agar (if needed) 18 g Distilled water 900 ml Add vitamin mix A1 after sterilization and cooling down at 50C. * Humic soil 1000 g Distilled water 1000 ml After sterilization, collect liquid portion by filtration/centrifigation. Soil extract can be stored at 4C for further use. CaCl2.2H2O 4 g ZnSO4.7H2O 2 g Na2B4O7.10H2O 0.1 g FeSO4. 7H2O 5 g KJ 0.05 g CoCl2.6H2O 0.5 g CuSO4.5H2O 0.2 g MnCl2.4H2O 2 g Na2MoO4.2H2O 0.05 g H2SO4 95 - 97 % p.a. 1 ml Demineralized water 1000 ml *p-aminobenzoic acid 0.05 g Calcium pantothenate 0.05 g Inositol 0.05 g Niacin 0.05 g Pyridoxin HCl 0.05 g Riboflavin 0.05 g Thiamine HCl 0.05 g Biotin 0.025 g Demineralized water 100 ml Sterilize by filtration
Growth temperature	28 °C
Oxygen requirement	Aerobic
Distribution condition	TBRC MTA
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Overseas: 3,000 Baht

Thailand: 2,000 Baht

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